Cloning and secretory expression of VP2 gene of infectious bursal disease virus in eukaryotic cells
نویسندگان
چکیده مقاله:
VP2 gene coding region of a vaccinal strain (D78) of infectious bursal disease virus (IBDV) was clonedin a eukaryotic expression vector, pSec Tag2A. The gene was placed downstream of Ig κ chain leadersequence, under the control of human cytomegalovirus (hCMV) immediate early enhancer and promoter. Theconstruct pSec Tag2A-VP2 was transfected in COS-7 cell line and the expression and secretion of VP2 wasassessed by dot blotting and antigen capture ELISA. The antibody used in the immunological assays was aneutralizing monoclonal antibody (1A6) against VP2. Positive reaction with the antibody indicated theconstruct was functional with respect to expression and secretion of a native VP2.
منابع مشابه
Cloning and Expression of VP2 Gene of Infectious Bursal Disease Virus in Eukaryotic Cells
Infectious bursal disease (IBD) is an economically important viral disease of chickens with worldwide distribution which suppresses the immune system of young chickens. VP2 is the major host-protective protein of infectious bursal disease virus (IBDV). The encoding region of VP2 protein was PCR amplified from a plasmid containing a cDNA fragment of large genomic segment of IBDV, strain D78. Thi...
متن کاملcloning and secretory expression of vp2 gene of infectious bursal disease virus in eukaryotic cells
vp2 gene coding region of a vaccinal strain (d78) of infectious bursal disease virus (ibdv) was clonedin a eukaryotic expression vector, psec tag2a. the gene was placed downstream of ig κ chain leadersequence, under the control of human cytomegalovirus (hcmv) immediate early enhancer and promoter. theconstruct psec tag2a-vp2 was transfected in cos-7 cell line and the expression and secretion of...
متن کاملcloning and expression of vp2 gene of infectious bursal disease virus in eukaryotic cells
infectious bursal disease (ibd) is an economically important viral disease of chickens with worldwide distribution which suppresses the immune system of young chickens. vp2 is the major host-protective protein of infectious bursal disease virus (ibdv). the encoding region of vp2 protein was pcr amplified from a plasmid containing a cdna fragment of large genomic segment of ibdv, strain d78. thi...
متن کاملCloning and expression of VP2 gene of Infectious bursal disease virus in eukaryotic cells
246 Abstract Infectious bursal disease (IBD) is an economically important viral disease of chickens with worldwide distribution which suppresses the immune system of young chickens. VP2 is the major host-protective protein of infectious bursal disease virus (IBDV). The encoding region of VP2 protein was PCR amplified from a plasmid containing a cDNA fragment of large genomic segment of IBDV, st...
متن کاملCloning and Codon-optimized Expression of Structural Protein Hypervariable Region of VP2 from Infectious Bursal Disease Virus
Infectious bursal disease virus (IBDV) is the causative agent of Gumboro disease, an infectious disease of global economic importance in poultry. Structural protein VP2 of IBDV is the most frequently studied protein due to its significant roles in virus attachment, protective immunity, and serotype specificity. The objective of the present study was to improve the expression of hypervariable re...
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Infectious bursal disease (IBD), a highly contagious and devastating disease in young chicken, is causedby infectious bursal disease virus (IBDV). To improve the immunogenicity of recombinant IBDV subunitvaccine, an attempt was made to find a new way to prepare IBD vaccine containing glycosylated mVP2antigen. Firstly, IBDV mVP2 gene (with a nucleic acid sequence encoding B cell epitope of IBDV(...
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عنوان ژورنال
دوره 11 شماره 1
صفحات 72- 77
تاریخ انتشار 2010-03-20
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